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This research was financially supported by the Carlos III Health Institute (Grant: PI 11/01634), and by grant RD12/0013/00010 from the Spanish Research Network on Adverse Reactions to Allergens and Drugs (RIRAAF; Red de Investigacion de Reacciones Adversas a Alergenos y Farmacos) of the Carlos III Health Institute.

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Is the ISAC 112 Microarray Useful in the Diagnosis of Pollinosis in Spain?

Publicated to:Journal Of Investigational Allergology And Clinical Immunology. 26 (2): 92-99 - 2016-01-01 26(2), DOI: 10.18176/jiaci.0052

Authors: Garcia, B. E.; Martinez-Aranguren, R.; Bernard Alonso, A.; Gamboa, P.; Feo Brito, F.; Bartra, J.; Blanca-Lopez, N.; Gomez, F.; Alvarado, M., I; Fernandez, J.; Alonso, M. D.; Gonzalez-Mancebo, E.; Moya, C.; Parra, A.; Terrados, S.; Sola, L.; Goikoetxea, M. J.; Sanz, M. L.;

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Abstract

Multiple sensitization is frequent among pollen-allergic patients. The goal of this study was to determine the diagnostic accuracy of the ImmunoCAP ISAC 112 (ISAC112) microarray in allergy to pollen from several taxa and its clinical utility in a Spanish population.Specific IgE was determined in 390 pollen-allergic patients using the ISAC112 microarray. Diagnostic accuracy (sensitivity, specificity, predictive values, and area under the ROC curve) was calculated for the diagnosis of allergy to pollen from grass (n=49), cypress (n=75), olive tree (n=33), plane tree (n=63), and pellitory of the wall (n=17) and compared with that of the singleplex ImmunoCAP immunoassay.The sensitivity of the ISAC112 microarray ranged from 68.2% for allergy to plane tree pollen to 93.9% for allergy to grass pollen. The specificity was >90%. The AUC for the diagnosis of allergy to plane tree pollen was 0.798, whereas the AUC for the remaining cases was ?0.876. The accuracy of ISAC112 was higher than that of ImmunoCAP for plane tree pollen and similar for the remaining pollens. The frequency of sensitization to most species-specific allergenic components and profilins varied between the different geographical regions studied. A total of 73% of pollen-allergic patients were sensitized to species-specific components of more than 1 pollen type.The ISAC112 microarray is an accurate tool for the diagnosis of allergy to pollen from grass, cypress, olive tree, plane tree, and pellitory of the wall. The features of the ISAC112 microarray are similar or superior (in the case of plane tree pollen) to those of ImmunoCAP. This microarray is particularly useful for the etiologic diagnosis of pollinosis in patients sensitized to multiple pollen species whose pollination periods overlap.

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