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March 14, 2017
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Biomarkers of spontaneous preterm birth: a systematic review of studies using multiplex analysis

Publicated to: Journal Of Perinatal Medicine. 45 (1): 71-84 - 2017-01-01 45(1), DOI: 10.1515/jpm-2016-0097

Authors:

Polettini, Jossimara; Cobo, Teresa; Kacerovsky, Marian; Vinturache, Angela E; Vinturache, Angela E; Laudanski, Piotr; Peelen, Myrthe J C S; Peelen, Myrthe J C S; Helmer, Hanns; Lamont, Ronald F; Lamont, Ronald F; Takeda, Jun; Lapointe, Jerome; Torloni, Maria Regina; Zhong, Nanbert; Menon, Ramkumar
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Affiliations

Acad Med Ctr, Dept Obstet & Gynecol, Amsterdam, Netherlands      University of Amsterdam    Academic Medical Center Amsterdam - Author
Charles Univ Prague, Dept Obstet & Gynecol, Fac Med Hradec Kralove, Univ Hosp Hradec Kralove, CR-11636 Prague 1, Czech Republic      Charles University Prague - Author
Hologic, Sunnyvale, CA USA - Author
Juntendo Univ, Fac Med, Dept Obstet & Gynecol, Tokyo, Japan      Juntendo University       - Author
Med Univ Bialystok, Dept Perinatol & Obstet, Bialystok, Poland      Medical University of Bialystok - Author
Med Univ Vienna, Dept Obstet & Gynecol, Vienna, Austria      Medical University of Vienna - Author
New York State Inst Basic Res Dev Disabil, 1050 Forest Hill Rd, Staten Isl, NY 10314 USA      Institute for Basic Research in Developmental Disabilities - Author
UCL, Div Surg, Northwick Pk Inst Med Res Campus, London, England      University College London    University of London - Author
Univ Barcelona, Inst Biomed Invest August Pi i Sunyer IDIBAPS, Maternal Fetal Med Dept, Hosp Clin, Barcelona, Spain      IDIBAPS    University of Barcelona    Hospital Clinic de Barcelona - Author
Univ Calgary, Cumming Sch Med, Dept Pediat, Calgary, AB, Canada      University of Calgary - Author
Univ Fed Sao Paulo, UNIFESP, Dept Obstet, Sao Paulo, SP, Brazil      Universidade Federal de Sao Paulo (UNIFESP) - Author
Univ Hosp Hradec Kralove, Biomed Res Ctr, Hradec Kralove, Czech Republic      Charles University Prague - Author
Univ Southern Denmark, Odense Univ Hosp, Dept Gynecol & Obstet, Inst Clin Res,Res Unit Gynecol & Obstet, Odense, Denmark      Odense University Hospital    University of Southern Denmark - Author
Univ Texas Med Branch, Dept Obstet & Gynecol, Div Maternal Fetal Med & Perinatal Res, MRB, 301 Univ Blvd,Room 11-138, Galveston, TX 77555 USA.      University of Texas Medical Branch Galveston    University of Texas System - Author
Univ Texas Med Branch, Dept Obstet & Gynecol, Div Maternal Fetal Med & Perinatal Res, MRB, 301 Univ Blvd,Room 11-138, Galveston, TX 77555 USA      University of Texas System    University of Texas Medical Branch Galveston - Author
Univ Western Sao Paulo, UNOESTE, Fac Hlth Sci, Sao Paulo, Brazil      Universidade do Oeste Paulista - Author
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Abstract

Despite decades of research on risk indicators of spontaneous preterm birth (PTB), reliable biomarkers are still not available to screen or diagnose high-risk pregnancies. Several biomarkers in maternal and fetal compartments have been mechanistically linked to PTB, but none of them are reliable predictors of pregnancy outcome. This systematic review was conducted to synthesize the knowledge on PTB biomarkers identified using multiplex analysis.Three electronic databases (PubMed, EMBASE and Web of Science) were searched for studies in any language reporting the use of multiplex assays for maternal biomarkers associated with PTB published from January 2005 to March 2014.Retrieved citations (3631) were screened, and relevant studies (33) were selected for full-text reading. Ten studies were included in the review. Forty-two PTB-related proteins were reported, and RANTES and IL-10 (three studies) followed by MIP-1?, GM-CSF, Eotaxin, and TNF-RI (two studies) were reported more than once in maternal serum. However, results could not be combined due to heterogeneity in type of sample, study population, assay, and analysis methods.By this systematic review, we conclude that multiplex assays are a potential technological advancement for identifying biomarkers of PTB, although no single or combination of biomarkers could be identified to predict PTB risk.
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Keywords

Amniotic cavityBiological markersBiomarkersCervical-fluidsCytokine levelsCytokinesDeliveryFemaleHumansInflammatory responseLabor analysisMaternal serumMicrobial invasionMultiplex assayPredictionPregnancyPremature birthPrematurityProteins

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal JOURNAL OF PERINATAL MEDICINE due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2017, it was in position , thus managing to position itself as a Q2 (Segundo Cuartil), in the category Pediatrics, Perinatology and Child Health. Notably, the journal is positioned en el Cuartil Q3 for the agency WoS (JCR) in the category Pediatrics.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 1.83. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 13, 2025)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Weighted Average of Normalized Impact by the Scopus agency: 1.01 (source consulted: FECYT Mar 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2026-04-25, the following number of citations:

  • WoS: 37
  • Scopus: 21
  • Europe PMC: 4
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-25:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 73.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 70 (PlumX).
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Austria; Brazil; Canada; Czech Republic; Denmark; Japan; Netherlands; Poland; United Kingdom; United States of America.

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Awards linked to the item

This study and report is made possible by Grant support from Hologic, Inc., CA, USA to PREBIC Biomarker Working Group (PI - Menon R) that helped us to organize PREBIC biomarker working group meetings in Barcelona, Spain (December, 2014) and PREBIC Annual Meeting in Florence, Italy (May, 2015) where the idea for this report was conceived, drafted and finalized.
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