{rfName}
Id

Indexed in

License and use

Icono OpenAccess

Altmetrics

Analysis of institutional authors

Carrascal MAuthor

Share

February 7, 2021
Publications
>
Article

Identification of promiscuous African swine fever virus t-cell determinants using a multiple technical approach

Publicated to:Vaccines. 9 (1): 29- - 2021-01-01 9(1), DOI: 10.3390/vaccines9010029

Authors: Bosch-Camós L; López E; Navas MJ; Pina-Pedrero S; Accensi F; Correa-Fiz F; Park C; Carrascal M; Domínguez J; Salas ML; Nikolin V; Collado J; Rodríguez F

Affiliations

Boehringer Ingelheim Veterinary Research Center GmbH and Co. KG - Author
Centre Recerca en Sanitat Animal - Author
Consejo Superior de Investigaciones Científicas - Author
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria - Author
Konkuk University - Author
OIE Collaborating Centre for the Research and Control of Emerging and Re-emerging Swine Diseases in Europe (IRTA-CReSA) - Author
Sanitat Animal. Malalties Exòtiques. IRTA Investigación y Tecnología Agroalimentarias - Author
Universitat Autònoma de Barcelona - Author
‎ Boehringer Ingelheim Vet Res Ctr BIVRC GmbH & Co, D-30559 Hannover, Germany - Author
‎ CSIC, Ctr Biol Mol Severo Ochoa, Madrid 28049, Spain - Author
‎ CSIC, Inst Invest Biomed Barcelona, Unidad Espectrometria Masas Biol & Proteom, Bellaterra 08193, Spain - Author
‎ Dept Biol Cellular Fisiol & Immunol, Campus Univ Autonoma Barcelona, Bellaterra 08193, Spain - Author
‎ Inst Nacl Invest & Tecnol Agr & Alimentaria INIA, Dept Biotecnol, Madrid 28049, Spain - Author
‎ IRTA, Ctr Recerca Sanitat Anim CReSA, Campus Univ Autonoma Barcelona, Bellaterra 08193, Spain - Author
‎ IRTA, OIE Collaborating Ctr Res & Control Emerging & Re, CReSA, Bellaterra 08193, Spain - Author
‎ Konkuk Univ, Dept Stem Cells & Regenerat Biol, Seoul 05029, South Korea - Author
‎ UAB, Fac Vet, Dept Sanitat & Anat Anim, Bellaterra 08193, Spain - Author
‎ UAB, IRTA, Ctr Recerca Sanitat Anim CReSA, Campus Univ Autonoma Barcelona, Bellaterra 08193, Spain - Author
‎ Univ Autonoma Madrid, Madrid 28049, Spain - Author
See more

Abstract

© 2021 by the authors. Licensee MDPI, Basel, Switzerland. The development of subunit vaccines against African swine fever (ASF) is mainly hindered by the lack of knowledge regarding the specific ASF virus (ASFV) antigens involved in protection. As a good example, the identity of ASFV-specific CD8+ T-cell determinants remains largely unknown, despite their protective role being established a long time ago. Aiming to identify them, we imple-mented the IFNγ ELISpot as readout assay, using as effector cells peripheral blood mononuclear cells (PBMCs) from pigs surviving experimental challenge with Georgia2007/1. As stimuli for the ELISpot, ASFV-specific peptides or full-length proteins identified by three complementary strategies were used. In silico prediction of specific CD8+ T-cell epitopes allowed identifying a 19-mer peptide from MGF100-1L, as frequently recognized by surviving pigs. Complementarily, the repertoire of SLA I-bound peptides identified in ASFV-infected porcine alveolar macrophages (PAMs), allowed the characterization of five additional SLA I-restricted ASFV-specific epitopes. Finally, in vitro stimulation studies using fibroblasts transfected with plasmids encoding full-length ASFV proteins, led to the identification of MGF505-7R, A238L and MGF100-1L as promiscuously recognized antigens. Interestingly, each one of these proteins contain individual peptides recognized by surviving pigs. Identification of the same ASFV determinants by means of such different approaches reinforce the results presented here.

Keywords

antigen presentationasfvcd8+ t-cellsepitope predictionsifn? elispotimmunopeptidomicsAntigen presentationAsfvCd8 t-cells +Cd8(+) t-cellsElispotEpitope predictionsIfn&#947Ifnγ elispotIm-munopeptidomicsImmunopeptidomicsPromiscuous epitope

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Vaccines due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2021, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Drug Discovery.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 5.39, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Aug 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-08-03, the following number of citations:

  • WoS: 7
  • Scopus: 1
  • Europe PMC: 15

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-08-03:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 26.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 28 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 1.
  • The number of mentions on the social network X (formerly Twitter): 2 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Germany; Republic of Korea.