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Analysis of institutional authors

Rodriguez-Garcia MAuthorFernández-Varo GAuthorMartínez IAuthorCasals ECorresponding AuthorMorales-Ruiz MAuthorCasals GCorresponding Author

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March 28, 2022
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Validation of a Microwave-Assisted Derivatization Gas Chromatography-Mass Spectrometry Method for the Quantification of 2-Hydroxybutyrate in Human Serum as an Early Marker of Diabetes Mellitus

Publicated to: Molecules. 27 (6): 1889- - 2022-03-01 27(6), DOI: 10.3390/molecules27061889

Authors: Rodríguez-García, M; Fernández-Varo, G; Hidalgo, S; Rodríguez, G; Martínez, I; Zeng, ML; Casals, E; Morales-Ruiz, M; Casals, G

Affiliations

Commiss Biochem Assessment Hepat Dis SEQCML, Barcelona 08036, Spain - Author
Inst Invest Biomed August Pi & Sunyer IDIBAPS, Ctr Invest Biomed Red Enfermedades Hepat & Digest, Hosp Clin Univ, Serv Biochem & Mol Genet, Barcelona 08036, Spain - Author
Univ Barcelona, Dept Biomed, Barcelona 08905, Spain - Author
Wuyi Univ, Sch Biotechnol & Hlth Sci, Jiangmen 529020, Peoples R China - Author
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Abstract

Circulating levels of 2-hydroxybutyrate (2HB) are highly related to glycemic status in different metabolomic studies. According to recent evidence, 2HB is an early biomarker of the future development of dysglycemia and type 2 diabetes mellitus and may be causally related to the progression of normal subjects to impaired fasting glucose or insulin resistance. In the present study, we developed and validated a simple, specific and sensitive gas chromatography-mass spectrometry (GC-MS) method specifically intended to quantify serum levels of 2HB. Liquid-liquid extraction with ethyl acetate was followed by 2 min of microwave-assisted derivatization. The method presented acceptable accuracy, precision and recovery, and the limit of quantification was 5 µM. Levels of 2HB were found to be stable in serum after three freeze-thaw cycles, and at ambient temperature and at a temperature of 4 °C for up to 24 h. Extracts derivatized under microwave irradiation were stable for up to 96 h. No differences were found in 2HB concentrations measured in serum or plasma EDTA samples. In summary, the method is useful for a rapid, precise and accurate quantification of 2HB in serum samples assessed for the evaluation of dysglycemia and diabetes mellitus.

Keywords

2-hydroxybutyrateaciddiabetes mellitusgc-mshuman seruminsulin-resistancemicrowave-assisted derivatizationtmstype-22-hydroxybutyrateBiochemical markersDiabetes mellitusDiabetis no-insulinodependentEarly metabolic markersGc-msHuman serumMarcadors bioquímicsMicrowave-assisted derivatizationNon-insulin-dependent diabetesTms

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Molecules due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2022, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Pharmaceutical Science.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-12-05:

  • WoS: 1
  • Scopus: 1

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-12-05:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 9 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: http://hdl.handle.net/2445/209325

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: China.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Rodriguez Garcia, Marta) and Last Author (Casals Mercadal, Gregori).

the authors responsible for correspondence tasks have been Casals Font, Elena and Casals Mercadal, Gregori.

Awards linked to the item

This research was funded by the Instituto de Salud Carlos III through the project PI19/00774 to G.F.-V. and G.C., co-funded by the European Regional Development Fund, European Union, A way to make Europe; Ministerio de Economia y Competitividad (PDI2019-105502RB-100 to MM-R); National Natural Science Foundation of China (31950410536 to E.C. and 22005221 to M.Z.); Wuyi University (2018TP010 to E.C., 2018TP011 and 2020FKZX05 to M.Z.) and Jiangmen City (2020JC01007 to M.Z.). CIBERehd is financed by the Instituto de Salud Carlos III.

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