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Analysis of institutional authors

Olle LAuthorGuo YAuthorMunoz-Cano RAuthorMartin MAuthor
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SARS-CoV-2 vaccine excipients polyethylene glycol and trometamol do not induce mast cell degranulation, in an in vitro model for non-IgE-mediated hypersensitivity

Publicated to:Frontiers In Allergy. 3 1046545-1046545 - 2022-12-20 3(), DOI: 10.3389/falgy.2022.1046545

Authors: Quan, Paola Leonor; Olle, Laia; Sabate-Bresco, Marina; Guo, Yanru; Munoz-Cano, Rosa; Wagner, Annette; Gastaminza, Gabriel; Martin, Margarita

Affiliations

ARADyAL, Res Network Asthma Drug Adverse React & Allergy Re, Madrid, Spain - Author
Biochemistry Unit, Biomedicine Department, Faculty of Medicine, University of Barcelona, Barcelona, Spain. - Author
Clin Univ Navarra, Dept Allergy & Clin Immunol, Pamplona, Spain - Author
Department of Allergy and Clinical Immunology, Clínica Universidad de Navarra, Pamplona, Spain. - Author
Guys & St ThomasNHS Fdn Trust, Dept Adult Allergy, London, England - Author
IDIBAPS, Lab Clin & Expt Resp Immunoallergy, Barcelona, Spain - Author
Inst Invest Sanit Navarra IdiSNa, Pamplona, Spain - Author
Instituto de Investigación Sanitaria de Navarra (IdiSNA), Pamplona, Spain. - Author
Laboratory of Clinical and Experimental Respiratory Immunoallergy, IDIBAPS, Barcelona, Spain. - Author
Univ Barcelona, Fac Med, Biomed Dept, Biochem Unit, Barcelona, Spain - Author
Univ Barcelona, Hosp Clin, Allergy Dept, Barcelona, Spain - Author
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Abstract

The development of vaccines against SARS-CoV2 brought about several challenges, including the management of hypersensitivity reactions to these formulations. The search for underlying mechanisms involved in these adverse events initially focused on excipients which may trigger mast cell activation responses via non-IgE pathways: polyethylene glycol and trometamol. We sought to determine whether these components, in their pure form, were capable of stimulating mast cells directly. To test this hypothesis, we used an in vitro model for non-IgE-mediated activation that has previously shown degranulation responses induced via MRGPRX2 with known drug agonists of the receptor. Human LAD2 mast cells were incubated with different concentrations (1, 10, 50 mg/ml) of trometamol and of purified polyethylene glycol/Macrogol (molecular weights: 2,000, 3,350, 4,000, and 6,000). Mast cell degranulation was assessed using a beta-hexosaminidase read-out. Interestingly, degranulation responses for all reagents tested showed no significant differences from those obtained from the negative control (basal degranulation). Receptor-silencing assays were therefore not conducted. In summary, purified PEG and trometamol did not induce mast cell degranulation in this in vitro model for the study of non-IgE mechanisms of drug hypersensitivity, previously shown to be useful in the investigation of MRGPRX2 ligands. Studies using complete vaccine formulations, lipid conjugates, and receptor gene variants are needed to further clarify mechanisms of vaccine hypersensitivity. 2022 Quan, Ollé, Sabaté-Brescó, Guo, Muñoz-Cano, Wagner, Gastaminza and Martín.

Keywords
Mast cellsMrgprx2Non-ige-mediated hypersensitivity mast cell activationPolyethylene glycolSars-cov-2 vaccinesTrometamol

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Frontiers In Allergy, Q4 Agency Scopus (SJR), its regional focus and specialization in Immunology and Allergy, give it significant recognition in a specific niche of scientific knowledge at an international level.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-05-17:

  • WoS: 1
  • Scopus: 1
  • Europe PMC: 1
  • OpenCitations: 1
Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-05-17:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 9.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 9 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 12.55.
  • The number of mentions on the social network X (formerly Twitter): 25 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: United Kingdom.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: Last Author (Martin Andorrà, Margarita).