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Grant support

This work was supported by the Ministry of Economy and Competition (MINECO), co-financed with the European Union FEDER funds (SAF2017-88606-P, 2017); SEPAR-AstraZeneca Ayudas Investigacion PII Oncologia 2021; Becas SEPAR 2022 (no de Proyecto 1326); Becas SEPAR 2023 (no de Proyecto 1482); and Catedra UB Hospital Clinico de Cancer de Pulmon Astrazeneca. Melissa Acosta-Plasencia is an FI-SDUR fellow from AGAUR.

Analysis of institutional authors

Martinez, DanielAuthorDiaz, TaniaAuthorMolins, LaureanoAuthorRamos, RicardAuthorMarrades, Ramon MAuthorNavarro, AlfonsCorresponding Author

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Article

Selection of the Most Suitable Culture Medium for Patient-Derived Lung Cancer Organoids

Publicated to:Cells Tissues Organs. - 2025-04-01 (), DOI: 10.1159/000541274

Authors: Acosta-Plasencia M; He Y; Martínez D; Orozco JP; Carrasco A; Altuna-Coy A; Yang T; Díaz T; Molins L; Ramos R; Marrades RM; Navarro A

Affiliations

Chengdu Univ, Sch Basic Med Sci, Chengdu, Peoples R China - Author
Hosp Clin Barcelona, Thorac Oncol Unit, Barcelona, Spain - Author
Inst Invest Biomed August Pi i Sunyer IDIBAPS, Barcelona, Spain - Author
Inst Salud Carlos III, Ctr Invest Biomed Red Enfermedades Respiratorias C, Madrid, Spain - Author
Univ Barcelona UB, Fac Med & Hlth Sci, Dept Surg & Med Specializat, Human Anat & Embryol Unit,Mol Oncol & Embryol Lab, Barcelona, Spain - Author
Univ Barcelona, Hosp Clin Barcelona, Dept Pathol, Barcelona, Spain - Author
Univ Barcelona, Hosp Clin Barcelona, Dept Thorac Surg, Barcelona, Spain - Author
Univ Barcelona, Hosp Clin Barcelona, Inst Clin Resp ICR, Dept Pneumol, Barcelona, Spain - Author
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Abstract

Introduction: Patient-derived organoids have emerged as a promising in vitro model for precision medicine, particularly in cancer, but also in noncancer-related diseases. However, the optimal culture medium for culturing patient-derived lung organoids has not yet been agreed upon. This study aimed to shed light on the optimal selection of a culture media for developing studies using patient-derived lung organoids. Methods: Tumor and normal paired tissue from 71 resected non-small cell lung cancer patients were processed for organoid culture. Lung cancer organoids (LCOs) were derived from tumor tissue and normal lung organoids (LNOs) from nonneoplastic lung tissue. Three different culture media were compared: permissive culture medium (PCM), limited culture medium (LCM), and minimum basal medium (MBM). We assessed their effectiveness in establishing organoid cultures, promoting organoid growth and viability, and compared their differential phenotypic characteristics. Results: While PCM was associated with the highest success rate and useful for long-term expansion, MBM was the best option to avoid normal organoid overgrowth in the organoid culture. The density, size, and viability of LNOs were reduced using LCM and severely affected with MBM. LNOs cultured in PCM tend to differentiate to bronchospheres, while alveolosphere differentiation can be observed in those cultured with LCM. The morphological phenotype of LCO was influenced by the culture media of election. Mesenchymal cell overgrowth was observed when LCM was used. Conclusion: This work highlights the importance of considering the research objectives when selecting the most suitable culture medium for growing patient-derived lung organoids.

Keywords

AlveolospheresBronchosphereBronchospheresCulture mediumDifferentiationLung cancer organoidsLung organoidsNon-small cell lung cancerResponseStem-cells

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Cells Tissues Organs due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2025, it was in position 132/205, thus managing to position itself as a Q1 (Primer Cuartil), in the category Cell Biology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-06-21:

  • Scopus: 2

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-21:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 7.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 7 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 9.08.
  • The number of mentions on the social network X (formerly Twitter): 2 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: China.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: Last Author (Navarro Ponz, Alfons).

the author responsible for correspondence tasks has been Navarro Ponz, Alfons.