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Lopez-Prades, SandraAuthorFuster-Anglada, CarlaAuthorFerrer-Fàbrega, JoanaAuthorBassegoda, OctaviAuthorBoix, LoretoAuthorCuatrecasas, MiriamAuthorDiaz, AlbaCorresponding Author
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Ex Vivo Fusion Confocal Microscopy of Liver Biopsies: Diagnostic Pattern Identification and Correlation with Conventional Microscopy

Publicated to:Applied Sciences-Basel. 14 (23): 11121- - 2024-12-01 14(23), DOI: 10.3390/app142311121

Authors: Lopez-Prades, Sandra; Fuster-Anglada, Carla; Millan, Rosana; Ferrer-Fabrega, Joana; Bassegoda, Octavi; Boix, Loreto; Cuatrecasas, Miriam; Diaz, Alba

Affiliations

Ctr Invest Biomed Red Enfermedades Hepat & Digest, Av Monforte Lemos 3-5, Madrid 28029, Spain - Author
Hosp Clin Barcelona, Dept Surg, Hepatobiliopancreat Surg & Liver & Pancreat Transp, C-Villarroel 170, Barcelona 08036, Spain - Author
Hosp Clin Barcelona, Liver Unit, Liver ICU, C-Villarroel 170, Barcelona 08036, Spain - Author
Hosp Clin Barcelona, Pathol Dept, C-Villarroel 170, Barcelona 08036, Spain - Author
IDIBAPS, Barcelona Clin Liver Canc BCLC Grp, C-Rosello 149, Barcelona 08036, Spain - Author
Inst Invest Biomed August Pi i Sunyer IDIBAPS, C-Rosello 149, Barcelona 08036, Spain - Author
Univ Barcelona, Fac Med, C-Casanova 143, E-08036 Barcelona, Spain - Author
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Abstract

Ex vivo Fusion Confocal Microscopy (eFuCM) is a promising new technique for real-time histological diagnosis, requiring minimal tissue preparation and avoiding tissue waste. This study aimed to evaluate the feasibility of eFuCM in identifying key liver biopsy lesions and patterns, and to assess the impact of eFuCM reading experience on diagnostic accuracy. Twenty-three fresh liver biopsies were analyzed using eFuCM to produce H&E-like digital images, which were reviewed by two pathologists and compared with a conventional H&E diagnosis. The liver architecture was clearly visible on the eFuCM images. Pathologist 1, with no prior eFuCM experience, achieved a substantial agreement with the H&E diagnosis (kappa = 0.65), while Pathologist 2, with eFuCM experience, reached almost perfect agreement (kappa = 0.88). However, lower agreement levels were found in the evaluation of inflammation. Importantly, tissue preparation for eFuCM did not compromise subsequent conventional histological processing. These findings suggest that eFuCM has great potential as a time- and material-saving tool in liver pathology, though its diagnostic accuracy improves with pathologist experience, indicating that there is a learning curve related to its use.

Keywords
Ex vivo fusion confocal microscopyFluorescence microscopyLiver pathologyMinimal sample preparationRapid evaluatioTime-efficient diagnosi

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Applied Sciences-Basel due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 258/439, thus managing to position itself as a Q1 (Primer Cuartil), in the category Materials Science, Multidisciplinary.

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-05-02:

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

    It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

    • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
    Leadership analysis of institutional authors

    There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (López Prades, Sandra) and Last Author (Díaz Lorca, Mª Alba).

    the author responsible for correspondence tasks has been Díaz Lorca, Mª Alba.