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Grant support

This work was supported by Ministerio de Ciencia e Innovacion Grant SAF 2007-60406 (to E. Vila) and Generalitat de Catalunya Grant 2009SGR-890. A. Marquez-Martin was supported by Universitat Autonoma de Barcelona.

Analysis of institutional authors

Marquez-Martin, AnaAuthorDantas, Ana PAuthorPlanas, Anna MAuthorVila, ElisabetCorresponding Author

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February 23, 2025
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Middle cerebral artery alterations in a rat chronic hypoperfusion model

Publicated to:Journal Of Applied Physiology. 112 (3): 511-518 - 2012-02-01 112(3), DOI: 10.1152/japplphysiol.00998.2011

Authors: Marquez-Martin, Ana; Jimenez-Altayo, Francesc; Dantas, Ana P; Caracuel, Laura; Planas, Anna M; Vila, Elisabet

Affiliations

Inst Invest Biomed August Pi & Sunyer, Dept Isquimia Cerebral & Neurodegeneracio, Barcelona, Spain - Author
Inst Invest Biomed August Pi & Sunyer, Inst Clin Torax, Barcelona, Spain - Author
Univ Autonoma Barcelona, Dept Farmacol Terapeut & Toxicol, Fac Med, Inst Neurociencies, Bellaterra 08193, Spain - Author

Abstract

Marquez-Martin A, Jimenez-Altayo F, Dantas AP, Caracuel L, Planas AM, Vila E. Middle cerebral artery alterations in a rat chronic hypoperfusion model. J Appl Physiol 112: 511-518, 2012. First published November 17, 2011; doi: 10.1152/japplphysiol.00998.2011.-Chronic cerebral hypoperfusion (CHP) induces microvascular changes that could contribute to the progression of vascular cognitive impairment and dementia in the aging brain. This study aimed to analyze the effects of CHP on structural, mechanical, and myogenic properties of the middle cerebral artery (MCA) after bilateral common carotid artery occlusion (BCCAO) in adult male Wistar rats. Sham animals underwent a similar surgical procedure without carotid artery (CA) ligation. After 15 days of occlusion, MCA and CA were dissected and MCA structural, mechanical, and myogenic properties were assessed by pressure myography. Collagen I/III expression was determined by immunofluorescence in MCA and CA and by Western blot in CA. mRNA levels for 1A1, 1A2, and 3A1 collagen subunits were quantified by quantitative real-time PCR in CA. Matrix metalloproteinase (MMP-1, MMP-2, MMP-9, and MMP-13) and hypoxia-inducible factor-1 alpha (HIF-1 alpha) protein expression were determined in CA by Western blot. BCCAO diminished cross-sectional area, wall thickness, and wall-to-lumen ratio. Nevertheless, whereas wall stress was increased, stiffness was not modified and myogenic response was diminished. Hypoperfusion triggered HIF-1 alpha expression. Collagen I/III protein expression diminished in MCA and CA after BCCAO, despite increased mRNA levels for 1A1 and 3A1 collagen subunits. Therefore, the reduced collagen expression might be due to proteolytic degradation, since the expression of MMP-1 and MMP-9 increased in the CA. These data suggest that BCCAO induces hypotrophic remodeling by a mechanism that involves a reduction of collagen I/III in association with increased MMP-1 and MMP-9 and that decreases myogenic tone in major arteries supplying the brain.

Keywords

Alzheimers-diseaseArteriolesBilateral common carotid artery occlusionBlood-flowCerebral blood flowDiseaseEssential-hypertensionFeaturesGenesHypoxiaInflammationInvolvementMatrixMetabolismMyogenic responsMyogenic responseMyogenic toneNuclear expressionOcclusionPatternPermanenReperfusionStageVascular remodelingWhite-matterZone

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Journal Of Applied Physiology due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2012, it was in position 7/84, thus managing to position itself as a Q1 (Primer Cuartil), in the category Sport Sciences.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 3.24, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Aug 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-08-02, the following number of citations:

  • WoS: 15
  • Scopus: 15
  • Europe PMC: 14
  • Google Scholar: 37

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-08-02:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 45 (PlumX).

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Marquez Cortes, Miguel Angel) and Last Author (Vila Navarro, Elena).

the author responsible for correspondence tasks has been Vila Navarro, Elena.