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This research was supported by the Agencia Estatal de Investigacion (PID2022-138243OB-I00 and MCIN/AEI/10.13039/501100011033/FEDER UE to MM-R), co-financed by FEDER, the European Union, "A way of making Europe"; Consolidated Research Group, Departament de Recerca i Universitats, Generalitat de Catalunya (2021 SGR 00881).

Analysis of institutional authors

De Medrano, Jaime I SainzAuthorLaguna, JavierAuthorFilella, XavierAuthorFernandez-Galan, EstherCorresponding Author

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Early Access

Comparison of two automated immunoassays for quantifying ProGRP, SCC and HE4 in serum: impact on diagnostic accuracy

Publicated to:Scandinavian Journal Of Clinical & Laboratory Investigation. 1-9 - 2025-02-14 (), DOI: 10.1080/00365513.2025.2466008

Authors: Sainz de Medrano JI; Laguna J; Julian J; Filella X; Fabregat A; Luquin M; Hurtado HH; García Humanes A; Morales-Ruiz M; Fernández-Galán E

Affiliations

Commiss Biochem Evaluat Hepat Dis SEQCML, Barcelona, Spain - Author
Ctr Invest Biomed Red Enfermedades Hepat & Digest, Madrid, Spain - Author
Hosp Clin Barcelona, Inst Invest Biomed August Pi i Sunyer FRCB IDIBAPS, Fundacio Recerca Clin Barcelona, Biochem & Mol Genet Dept CDB, 170 Villarroel St, Barcelona 08036, Spain - Author
Hosp Univ Navarra, Serv Anal Clin, Pamplona, Spain - Author
Univ Barcelona, Fac Med & Hlth Sci, Biomed Dept, Barcelona, Spain - Author
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Abstract

ProGRP (Progastrin-releasing peptide), SCC (Squamous Cell Carcinoma Antigen), and HE4 (Human epididymis protein 4) are serum tumor markers (STMs) frequently used in clinical practice, particularly for detection and monitoring of ovarian and lung neoplasms. In clinical laboratories, their quantification is commonly performed using automated immunoassays. Nevertheless, variations in results obtained by different immunoassays can impact diagnostic accuracy and effectiveness of patient monitoring. Our aim is to assess differences in STMs concentrations between two automated immunoassays: the Elecsys (Roche) and the Architect (Abbott), which are integrated into the Cobas e402 and Architect i2000SR systems respectively. We included 401 serum samples from patients with different clinical conditions: patients with cancer (n = 170), benign diseases (n = 100) and a control group (n = 131). ProGRP, SCC, and HE4 concentrations were quantified in parallel by both analyzers. To evaluate the clinical impact of changing these methodologies, overall concordance, the kappa index and ROC (Receiver Operating Characteristic) curves were calculated. While some discrepancies were noted in specific cases, overall, we obtained a good correlation for three STMs, with a Pearson coefficient for proGRP (r = 0.99), SCC (r = 0.95) and HE4 (r = 0.973). We also obtained a similar performance in the differential diagnosis of cancer, according to the results of the ROC analyses for Cobas and Archictect assays respectively: proGRP (AUC = 0.92; 0.91), SCC (AUC = 0.90; 0.92) and HE4 (AUC = 0.92; 0.93).

Keywords

BenigCancerCarcinoma antigens 1Comparative studyExpressionGastrin-releasing-peptideHe4ImmunoassayProgrpSccTumor markerTumor markersTumor-markers

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Scandinavian Journal Of Clinical & Laboratory Investigation due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2025, it was in position , thus managing to position itself as a Q2 (Segundo Cuartil), in the category Medicine (Miscellaneous). Notably, the journal is positioned en el Cuartil Q4 for the agency WoS (JCR) in the category Medicine, Research & Experimental.

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-16:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 2 (PlumX).

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Sainz de Medrano Sainz, Jaime Ignacio) and Last Author (Fernández Galán, Esther).

the author responsible for correspondence tasks has been Fernández Galán, Esther.