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Pascal, MarionaAuthor

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May 13, 2025
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Article

Comparative Assessment of the Allergenicity of Hyaluronidases from Polistes dominula (Pol d 2), Vespula vulgaris (Ves v 2), and Apis mellifera Venom (Api m 2)

Publicated to: Toxins. 16 (11): 498- - 2024-11-01 16(11), DOI: 10.3390/toxins16110498

Authors:

Grosch, Johannes; Eberlein, Bernadette; Waldherr, Sebastian; Pascal, Mariona; Dorn, Britta; San Bartolome, Clara; Pinzon, Federico De La Roca; Schiener, Maximilian; Darsow, Ulf; Biedermann, Tilo; Lidholm, Jonas; Bilo, Maria Beatrice; Jakob, Thilo; Schmidt-Weber, Carsten B; Blank, Simon
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Affiliations

Helmholtz Munich, German Res Ctr Environm Hlth, D-85764 Munich, Germany - Author
Hosp Clin Barcelona, Allergy Sect, Clin Med Grp, Barcelona 08036, Spain - Author
Ist Nazl Riposo & Cura Anziani INCRA, Ist Ricovero & Cura Carattere Sci IRCCS, I-60121 Ancona, Italy - Author
Justus Liebig Univ Giessen, Dept Dermatol & Allergol, Expt Dermatol & Allergy Res Grp, Giessen, Germany - Author
Polytech Univ Marche, Dept Clin & Mol Sci, I-60121 Ancona, Italy - Author
Spanish Network Allergy RETIC Asma Reacciones Adv, Madrid 28040, Spain - Author
Tech Univ Munich, Sch Med & Hlth, Ctr Allergy & Environm ZAUM, D-85764 Munich, Germany - Author
Tech Univ Munich, Sch Med & Hlth, Dept Dermatol & Allergy Biederstein, D-80802 Munich, Germany - Author
Thermo Fisher Sci, S-75450 Uppsala, Sweden - Author
Univ Barcelona, CDB Hosp Clin Barcelona, Immunol Dept, Barcelona 08007, Spain - Author
Univ Barcelona, Inst Invest Biomed August Pi Sunyer IDIBAPS, CDB Hosp Clin Barcelona, Immunol Dept, Barcelona 08007, Spain - Author
Univ Hosp Ancona, Dept Internal Med, Allergy Unit, I-60126 Ancona, Italy - Author
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Abstract

Sensitization to cross-reactive allergens complicates identifying the culprit insect in Hymenoptera venom allergy via diagnostic tests. This study evaluates sensitization to hyaluronidases (Api m 2 from honey bee (Apis mellifera) venom, HBV; Pol d 2 from European paper wasp (Polistes dominula) venom, PDV; and Ves v 2.0101 and Ves v 2.0201 from yellow jacket (Vespula vulgaris) venom, YJV) and their cross-reactivity in allergic patients from Italy, Spain, and Germany using ImmunoCAPs, ELISA, and basophil activation tests. Sensitization rates were 45% for Api m 2 in HBV-allergic subjects, 25% for Pol d 2 in PDV-allergic individuals, and 20% and 10% for Ves v 2.0201 and Ves v 2.0101 in YJV-allergic patients, respectively. Patients primarily sensitized to Api m 2 showed minimal cross-reactivity to vespid hyaluronidases, whereas those primarily sensitized to Pol d 2 or Ves v 2.0201 exhibited IgE reactivity to Api m 2. Neither Pol d 2 nor Ves v 2.0201 triggered basophil activation. Cross-reactivity of Api m 2, Pol d 2, and Ves v 2.0201 depends on the primary sensitizing venom. Sensitization to Pol d 2 and Ves v 2.0201 remains below 25%, yet these patients may exhibit cross-reactivity to Api m 2. Conversely, HBV-allergic patients sensitized to Api m 2 show minimal reactivity to Pol d 2 or Ves v 2.0201.
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Keywords

AdolescentAdultAgedAllergen cross-reactivityAllergensAnimalsApi m 2Apis melliferaBeBee venomsBeesChildCross reactionsFemaleHumansHyaluronidasesHymenopteraHymenoptera venom allergyHypersensitivityIgeImmunoglobulin eInsect bites and stingsInsect proteinsMaleMiddle agedPol d 2Polistes dominulaReactive carbohydrate determinantsRecombinantVes v 2Vespula vulgariVespula vulgarisWasp venomsWaspsYoung adult

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Toxins due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 26/106, thus managing to position itself as a Q1 (Primer Cuartil), in the category Toxicology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-05:

  • WoS: 3
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-05:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 7 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

    It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

    • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
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    Leadership analysis of institutional authors

    This work has been carried out with international collaboration, specifically with researchers from: Germany; Italy; Sweden.

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    Awards linked to the item

    This work was supported by the Helmholtz Association, Future Topic "Immunology and Inflammation" (ZT-0027) to S.B. and C.B.S.-W.
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