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Grant support

We acknowledge the funding from Advanced Marker Discovery (AMADIX) and grants support from Fundacion Cientifica de la Asociacion Espanola contra el Cancer (GCB13131592CAST), Ministerio de Economia y Competitividad (RTC-2015-3850-1 and SAF2014-54453-R) and Instituto de Salud Carlos III (PI17/01003, co-funded by FEDER-European Union). CIBEREHD is funded by the Instituto de Salud Carlos III. We are grateful to the support of CERCA Programme and SGR 653 from Generalitat de Catalunya. This work was developed at the Centro Esther Koplowitz, Barcelona, Spain.

Analysis of institutional authors

Duran-Sanchon SAuthorVila-Navarro EAuthorMarcuello MAuthorDiez MsAuthorHernandez VAuthorMartín AcAuthorCastells AAuthorGironella MCorresponding Author
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Article

Validation of miR-1228-3p as Housekeeping for MicroRNA Analysis in Liquid Biopsies from Colorectal Cancer Patients

Publicated to:Biomolecules. 10 (1): E16- - 2020-01-01 10(1), DOI: 10.3390/biom10010016

Authors: Duran-Sanchon, Saray; Vila-Navarro, Elena; Marcuello, Maria; Jose Lozano, Juan; Munoz, Jenifer; Cubiella, Joaquin; Soledad Diez, Maria; Bujanda, Luis; Lanas, Angel; Jover, Rodrigo; Hernandez, Vicent; Quintero, Enrique; Herreros-Villanueva, Marta; Carmen Martin, Ana; Perez-Palacios, Rosa; Arroyo, Rocio; Castells, Antoni; Gironella, Meritxell

Affiliations

Adv Marker Discovery AMADIX, Acera Recoletos 2, Valladolid 47004, Spain - Author
CIBEREHD, Bioinformat Platform, Barcelona 08036, Catalonia, Spain - Author
Complexo Hosp Univ Ourense, Inst Invest Sanitaria Galicia Sur, CIBEREHD, Dept Gastroenterol, Orense 32005, Spain - Author
Complexo Hosp Univ Vigo, Dept Gastroenterol, Vigo 36214, Spain - Author
Ctr Invest Biomed Canarias CIBICAN, Tenerife 38320, Spain - Author
Hosp Gen Univ Alicante, Gastroenterol Unit, Alicante 03010, Spain - Author
Hosp Univ Burgos, Dept Gastroenterol, Burgos 09006, Spain - Author
Univ Barcelona, Inst Invest Biomed August Pi & Sunyer IDIBAPS, Ctr Invest Biomed Red Enfermedades Hepat & Digest, Gastrointestinal & Pancreat Oncol Grp,Hosp Clin B, E-08036 Barcelona, Catalonia, Spain - Author
Univ La Laguna, Inst Univ Tecnol Biomed ITB, Hosp Univ Canarias, Dept Gastroenterol, E-38320 Tenerife, Spain - Author
Univ Pais Vasco UPV EHU, Ctr Invest Biomed Red Enfermedades Hepat & Digest, Dept Gastroenterol, Hosp Donostia,Inst Biodonostia, San Sebastian 20014, Spain - Author
Univ Zaragoza, CIBEREHD, Hosp Clin Univ, Dept Gastroenterol,IIS Aragon, E-50009 Zaragoza, Spain - Author
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Abstract

Circulating microRNA (miRNA) analysis is a growing research field. However, it usually requires an endogenous control or housekeeping (HK) in order to normalize expression of specific miRNAs throughout different samples. Unfortunately, no adequate HK for circulating miRNA analysis is still known in the colorectal cancer (CRC) context whereas several have been suggested. Hence, our aims were to validate the previously suggested miR-1228-3p as HK for CRC studies, to compare its suitability with the widely used miR-16-5p, and to evaluate the influence of hemolysis on both miRNAs.We analyzed by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) the expression of miR-1228-3p, miR-16-5p and the spike-in cel-miR-39 in a set of 297 plasmas (92 CRC, 101 advanced adenomas -AA-, and 100 controls) and 213 serum samples (59 CRC, 74 AA and 80 controls). We also analyzed both miRNAs depending on the hemolysis degree in 7 plasmas and 31 serums.Levels of miR-1228-3p and miR-16-5p did not show significant differences between groups although miR-16-5p exhibited more variability in plasma and serum samples. Importantly, the combination of cel-miR-39 and miR-1228-3p was the most stable one. Moreover, we observed that miR-16-5p was significantly influenced by hemolysis in contrast with miR-1228-3p that exhibited no correlation with this confounding factor in both biofluids.MiR-1228-3p has been validated as an adequate endogenous control for circulating miRNA analysis in CRC and AA liquid biopsies.

Keywords
colon cancerdiagnosisendogenous controlexpressiongenesmirnasnon-invasive diagnosisnormalizationpcrplasmapotential biomarkersprognosisqrt-pcrquantificationreference mirnaserumtumor markerAgedAged, 80 and overBiomarkerBiomarkers, tumorCirculating micrornasColon cancerColorectal neoplasmsDiagnosisEndogenous controlExpressionFemaleGene expression regulation, neoplasticGenesGenes, essentialHumansLiquid biopsyMaleMicrornasMiddle agedMirn1228 microrna, humanMirnasNon-invasive diagnosisNormalizationPcrPlasmaPotential biomarkersPrognosisQrt-pcrQuantificationReference mirnaSerumTumor marker

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Biomolecules due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2020, it was in position 96/295, thus managing to position itself as a Q2 (Segundo Cuartil), in the category Biochemistry & Molecular Biology. Notably, the journal is positioned en el Cuartil Q2 para la agencia Scopus (SJR) en la categoría Biochemistry.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 1.29, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions May 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-05-10, the following number of citations:

  • WoS: 9
  • Scopus: 12
  • Europe PMC: 8
  • OpenCitations: 9
Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-05-10:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 42.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 42 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 0.25.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Durán Sanchón, Saray) and Last Author (Gironella Cos, Meritxell).

the author responsible for correspondence tasks has been Gironella Cos, Meritxell.