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Analysis of institutional authors

Bielsa NAuthorEnrich CAuthor

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October 5, 2022
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Methuosis Contributes to Jaspine-B-Induced Cell Death

Publicated to: International Journal Of Molecular Sciences. 23 (13): 7257- - 2022-07-01 23(13), DOI: 10.3390/ijms23137257

Authors: Bielsa, N; Casasampere, M; Abad, JL; Enrich, C; Delgado, A; Fabriàs, G; Lizcano, JM; Casas, J

Affiliations

Departament de Biomedicina, Unitat de Biologia Cel·lular, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, Barcelona, 08036, Spain, Centre de Recerca Biomèdica CELLEX, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS - Author
Endocitosi i tràfic intracel·lular de proteïnes i colesterol. Institut d'Investigacions Biomèdiques August Pi i Sunyer - Author
Inst Adv Chem Catalonia IQAC CSIC, Dept Biol Chem, Res Unit BioAct Mol, Barcelona 08034, Spain - Author
Inst Invest Biomed August Pi i Sunyer IDIBAPS, Ctr Recerca Biomed CELLEX, Barcelona 08036, Spain - Author
ISCIII, Liver & Digest Dis Networking Biomed Res Ctr CIBE, Madrid 28029, Spain - Author
Protein Kinases and Signal Transduction Laboratory, Departament de Bioquímica i Biologia Molecular and Institut de Neurociències, Universitat Autònoma de Barcelona (UAB), Barcelona, 08193, Spain, Protein Kinases in Cancer Research, Vall Hebron Institut de - Author
Research Unit on BioActive Molecules, Department of Biological Chemistry, Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Barcelona, 08034, Spain - Author
Research Unit on BioActive Molecules, Department of Biological Chemistry, Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Barcelona, 08034, Spain, Department of Pharmacology, Toxicology and Medicinal Chemistry, Unit of Pharmaceutical Chemistry - Author
Research Unit on BioActive Molecules, Department of Biological Chemistry, Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Barcelona, 08034, Spain, Liver and Digestive Diseases Networking Biomedical Research Centre (CIBEREHD), ISCIII, Madrid, 28 - Author
Univ Autonoma Barcelona UAB, Dept Bioquim & Biol Mol, Prot Kinases & Signal Transduct Lab, Barcelona 08193, Spain - Author
Univ Autonoma Barcelona UAB, Inst Neurociencies, Barcelona 08193, Spain - Author
Univ Barcelona, Fac Med & Ciencies Salut, Dept Biomed, Unitat Biol Cellular, Barcelona 08036, Spain - Author
Univ Barcelona, Fac Pharm & Food Sci, Dept Pharmacol Toxicol & Med Chem, Unit Pharmaceut Chem,Associated Unit,CSIC, Barcelona 08028, Spain - Author
Vall Hebron Inst Recerca VHIR, Prot Kinases Canc Res, Barcelona 08193, Spain - Author
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Abstract

Methuosis is a type of programmed cell death in which the cytoplasm is occupied by fluidfilled vacuoles that originate from macropinosomes (cytoplasmic vacuolation). A few molecules have been reported to behave as methuosis inducers in cancer cell lines. Jaspine B (JB) is a natural anhydrous sphingolipid (SL) derivative reported to induce cytoplasmic vacuolation and cytotoxicity in several cancer cell lines. Here, we have investigated the mechanism and signalling pathways involved in the cytotoxicity induced by the natural sphingolipid Jaspine B (JB) in lung adenocarcinoma A549 cells, which harbor the G12S K-Ras mutant. The effect of JB on inducing cytoplasmic vacuolation and modifying cell viability was determined in A549 cells, as well as in mouse embryonic fibroblasts (MEF) lacking either the autophagy-related gene ATG5 or BAX/BAK genes. Apoptosis was analyzed by flow cytometry after annexin V/propidium iodide staining, in the presence and absence of z-VAD. Autophagy was monitored by LC3-II/GFP-LC3-II analysis, and autophagic flux experiments using protease inhibitors. Phase contrast, confocal, and transmission electron microscopy were used to monitor cytoplasmic vacuolation and the uptake of Lucifer yellow to assess macropinocyosis. We present evidence that cytoplasmic vacuolation and methuosis are involved in Jaspine B cytotoxicity over A549 cells and that activation of 5' AMP-activated protein kinase (AMPK) could be involved in Jaspine-B-induced vacuolation, independently of the phosphatidylinositol 3-kinase/protein kinase B/mechanistic target of rapamycin complex 1 (PI3K/Akt/mTORC1) axis. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

apoptosisautophagybiological evaluationcytoplasmic vacuolizationdiscoverydysfunctioninhibitionmacropinocytosismethuosispachastrissaminephosphorylationsphingolipidsvacuolizationActivated protein-kinaseAnimalAnimalsApoptosisAutophagyCell deathCell line, tumorCell survivalCytoplasmic vacuolizationEndosomeEndosomesFibroblastFibroblastsMammalian target of rapamycin complex 1Mechanistic target of rapamycin complex 1MethuosisMiceMouseNeoplasmNeoplasmsPachastrissaminePhosphatidylinositol 3 kinasePhosphatidylinositol 3-kinasesSphingolipidSphingolipidsSphingosineTumor cell line

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal International Journal Of Molecular Sciences due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2022, it was in position 66/285, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biochemistry & Molecular Biology.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 1.44. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 13, 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-12-12, the following number of citations:

  • WoS: 17
  • Scopus: 8
  • Europe PMC: 2

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-12-12:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 11 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

    It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

    • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

    Leadership analysis of institutional authors

    There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Bladé Creixenti, Joan) .