{rfName}

Indexed in

License and Use

Icono OpenAccess

Citations

25

Altmetrics

Analysis of institutional authors

Puerta-Alcalde, PedroAuthorSoriano, AlexAuthorSanz, SergiAuthor

Share

May 13, 2024
Publications
>
Article
Sí

An Assessment of a New Rapid Multiplex PCR Assay for the Diagnosis of Meningoencephalitis

Publicated to: Diagnostics. 14 (8): 802- - 2024-04-01 14(8), DOI: 10.3390/diagnostics14080802

Authors:

Cuesta, Genoveva; Puerta-Alcalde, Pedro; Vergara, Andrea; Roses, Enric; Bosch, Jordi; Casals-Pascual, Climent; Soriano, Alex; Marcos, M angeles; Sanz, Sergi; Vila, Jordi
[+]

Affiliations

Barcelona Inst Global Hlth ISGlobal, Barcelona, Spain - Author
Hosp Clin Barcelona, Dept Clin Microbiol, Ctr Biomed Diag, Barcelona 08036, Spain - Author
Inst Salud Carlos III, CIBER Enfermedades Infecciosas CIBERINFEC, Madrid, Spain - Author
Univ Barcelona, Hosp Clin, Dept Infect Dis, IDIBAPS, Barcelona 08007, Spain - Author
Univ Barcelona, Med Sch, Dept Basic Clin Pract, Barcelona 08007, Spain - Author
See more

Abstract

The rapid and broad microbiological diagnosis of meningoencephalitis (ME) has been possible thanks to the development of multiplex PCR tests applied to cerebrospinal fluid (CSF). We aimed to assess a new multiplex PCR panel (the QIAstat-Dx ME panel), which we compared to conventional diagnostic tools and the Biofire FilmArray ME Panel. The pathogens analyzed using both methods were Escherichia coli K1, Haemophilus influenzae, Listeria monocytogenes, Neisseria meningitidis, Streptococcus agalactiae, Streptococcus pneumoniae, Enterovirus, herpes simplex virus 1-2, human herpesvirus 6, human parechovirus, varicella zoster virus, and Cryptococcus neoformans/gattii. We used sensitivity, specificity, PPV, NPV, and kappa correlation index parameters to achieve our objective. Fifty CSF samples from patients with suspected ME were included. When conventional methods were used, 28 CSF samples (56%) were positive. The sensitivity and specificity for QIAstat-Dx/ME were 96.43% (CI95%, 79.8-99.8) and 95.24% (75.2-99.7), respectively, whereas the PPV and NPV were 96.43% (79.8-99.8) and 95.24% (75.1-99.7), respectively. The kappa value was 91.67%. Conclusions: A high correlation of the QIAstat-Dx ME panel with reference methods was shown. QIAstat-Dx ME is a rapid-PCR technique to be applied in patients with suspected ME with a high accuracy.
[+]

Keywords

AdultAgedArticleAseptic meningitisCerebrospinal fluid cytologyCerebrospinal-fluidClinical articleControlled studyCryptococcus neoformansDiagnostic test accuracy studyEncephalitisEnterovirusEpidemic meningitisEpilepsyEscherichia coliFalse negative resultFalse positive resultFilmarray meHaemophilus influenzaeHerpes simplex virus 2Herpes zoster encephalitisHerpetic encephalitisHumanHuman alphaherpesvirus 1Human herpesvirus 6Human parechovirusLimbic encephalitisLimit of detectionListeria monocytogenesListeriosisMe panelMelas syndromeMeningitisMeningococcemiaMeningoencephalitisMiddle agedMultiplex pcrMultiplex polymerase chain reactionNeisseria meningitidisNonhumanPneumococcal meningitisQiastat-dx meSensitivity and specificityStreptococcus agalactiaeStreptococcus pneumoniaeVaricella zoster virusVirus detectionWernicke encephalopathyYoung adult

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Diagnostics due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 57/332, thus managing to position itself as a Q1 (Primer Cuartil), in the category Medicine, General & Internal.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-01:

  • WoS: 9
  • Scopus: 2
[+]

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-01:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 18.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 17 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 1.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
[+]

Awards linked to the item

No Statement Available
[+]