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Grant support

The present work was supported by grants from Instituto de Salud Carlos III (PI10/02888 and PI13/02192, co-funded by FEDER-European Union) and Fundacion Mutua Madrilena to MG, and Fundacion Cientifica de la Asociacion Espanola contra el Cancer (GCB13131592CAST) and Ministerio de Economia y Competitividad (SAF2014-54453-R) to AC. CIBEREHD is funded by the Instituto de Salud Carlos III. This work was developed at the Centro Esther Koplowitz, Barcelona, Spain. MV-C is funded by Ministerio de Educacion Cultura y Deporte (FPU12/05138).

Analysis of institutional authors

Fernández Esparrach, GloriaAuthorVila-Navarro, EAuthorVila-Casadesús, MAuthorMoreira, LAuthorDuran-Sanchon, SAuthorSinha, RAuthorGines, AAuthorCuatrecasas, MAuthorCastells, AAuthorGironella, MCorresponding Author

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May 31, 2016
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MicroRNAs for Detection of Pancreatic Neoplasia: Biomarker Discovery by Next-generation Sequencing and Validation in 2 Independent Cohorts

Publicated to:Annals Of Surgery. 265 (6): 1226-1234 - 2017-06-01 265(6), DOI: 10.1097/SLA.0000000000001809

Authors: Vila-Navarro, Elena; Vila-Casadesus, Maria; Moreira, Leticia; Duran-Sanchon, Saray; Sinha, Rupal; Gines, Angels; Fernandez-Esparrach, Gloria; Miquel, Rosa; Cuatrecasas, Miriam; Castells, Antoni; Jose Lozano, Juan; Gironella, Meritxell

Affiliations

CIBEREHD Barcelona, Bioinformat Platform, Catalonia, Spain - Author
Hosp Clin Barcelona, Pathol Dept, Barcelona, Catalonia, Spain - Author
Univ Barcelona, Inst Invest Biomed August Pi & Sunyer IDIBAPS, Ctr Invest Biomed Red Enfermedades Hepat & Digest, Gastrointestinal & Pancreat Oncol Grp,Hosp Clin B, Barcelona, Catalonia, Spain - Author

Abstract

The aim of our study was to analyze the miRNome of pancreatic ductal adenocarcinoma (PDAC) and its preneoplastic lesion intraductal papillary mucinous neoplasm (IPMN), to find new microRNA (miRNA)-based biomarkers for early detection of pancreatic neoplasia.Effective early detection methods for PDAC are needed. miRNAs are good biomarker candidates.Pancreatic tissues (n = 165) were obtained from patients with PDAC, IPMN, or from control individuals (C), from Hospital Clínic of Barcelona. Biomarker discovery was done using next-generation sequencing in a discovery set of 18 surgical samples (11 PDAC, 4 IPMN, 3 C). MiRNA validation was carried out by quantitative reverse transcriptase PCR in 2 different set of samples. Set 1-52 surgical samples (24 PDAC, 7 IPMN, 6 chronic pancreatitis, 15 C), and set 2-95 endoscopic ultrasound-guided fine-needle aspirations (60 PDAC, 9 IPMN, 26 C).In all, 607 and 396 miRNAs were significantly deregulated in PDAC and IPMN versus C. Of them, 40 miRNAs commonly overexpressed in both PDAC and IPMN were selected for further validation. Among them, significant up-regulation of 31 and 30 miRNAs was confirmed by quantitative reverse transcriptase PCR in samples from set 1 and set 2, respectively.miRNome analysis shows that PDAC and IPMN have differential miRNA profiles with respect to C, with a large number of deregulated miRNAs shared by both neoplastic lesions. Indeed, we have identified and validated 30 miRNAs whose expression is significantly increased in PDAC and IPMN lesions. The feasibility of detecting these miRNAs in endoscopic ultrasound-guided fine-needle aspiration samples makes them good biomarker candidates for early detection of pancreatic cancer.

Keywords

biomarkerscancercarcinomadiagnosisductal adenocarcinomaeus-fnaexpressionipmnmir-155mirnapancreatic cancerrnastumor markertumorigenesisBiomarkers, tumorCarcinoma, pancreatic ductalCarcinoma, papillaryDeep sequencingEarly detection of cancerEus-fnaGene expression profilingHumansIpmnMicrornasMirnaPancreatic cancerPancreatic neoplasmsPapillary-mucinous neoplasmsPrecancerous conditionsReverse transcriptase polymerase chain reactionTumor marker

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Annals Of Surgery due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2017, it was in position 1/200, thus managing to position itself as a Q1 (Primer Cuartil), in the category Surgery. Notably, the journal is positioned above the 90th percentile.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 2.48. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 14, 2024)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Weighted Average of Normalized Impact by the Scopus agency: 1.98 (source consulted: FECYT Feb 2024)
  • Field Citation Ratio (FCR) from Dimensions: 13.14 (source consulted: Dimensions Aug 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-08-04, the following number of citations:

  • WoS: 47
  • Scopus: 36
  • Europe PMC: 34

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-08-04:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 74.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 96 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 7.2.
  • The number of mentions on the social network X (formerly Twitter): 11 (Altmetric).

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Vila Navarro, Elena) and Last Author (Gironella Cos, Meritxell).

the author responsible for correspondence tasks has been Gironella Cos, Meritxell.