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Grant support

This work was partly supported by grants from Generalitat de Catalunya (17SGR437), Gilead Sciences Fellowship (GLD17/00282), Ministerio de Educacion, Cultura y Deporte of Spain (FPU17/00361), Fundacion Espanola de Hematologia y Hemoterapia (FEHH-Janssen), Instituto de Salud Carlos III/FEDER (PT17/0015/0011) and the Xarxa de Bancs de tumors sponsored by Pla Director d'Oncologia de Catalunya (XBTC).

Analysis of institutional authors

Gimeno, ElenaAuthorBea, SAuthorOrtega MAuthorBlanco MlAuthorBaumann TAuthorBosch FAuthor

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Article

Chromosome banding analysis and genomic microarrays are both useful but not equivalent methods for genomic complexity risk stratification in chronic lymphocytic leukemia patients

Publicated to:Haematologica. 107 (3): 593-603 - 2022-03-01 107(3), DOI: 10.3324/haematol.2020.274456

Authors: Ramos-Campoy, Silvia; Puiggros, Anna; Bea, Silvia; Bougeon, Sandrine; Jose Larrayoz, Maria; Costa, Dolors; Parker, Helen; Matteo Rigolin, Gian; Ortega, Margarita; Laura Blanco, Maria; Collado, Rosa; Salgado, Rocio; Baumann, Tycho; Gimeno, Eva; Moreno, Carolina; Bosch, Francesc; Calvo, Xavier; Jose Calasanz, Maria; Cuneo, Antonio; Strefford, Jonathan C; Nguyen-Khac, Florence; Oscier, David; Haferlach, Claudia; Schoumans, Jacqueline; Espinet, Blanca

Affiliations

CIBERONC, Inst Invest Biomed August Pi & Sunyer IDIBAPS, Hematopathol Unit, Hosp Clin, Barcelona, Spain - Author
Consorcio Hosp Gen Univ, Dept Hematol, Valencia, Spain - Author
Fdn Jimenez Diaz, Hematol Dept, Cytogenet Lab, Madrid, Spain - Author
Hematol Dept, Paris, France - Author
Hosp del Mar, Pathol Dept, Mol Cytogenet Lab, Barcelona, Spain - Author
Hosp Santa Creu & Sant Pau, Dept Hematol, Barcelona, Spain - Author
IMIM Hosp del Mar, Canc Res Program, Appl Clin Res Hematol Malignances, Barcelona, Spain - Author
Inst Hosp Mar Invest Med IMIM, Canc Res Program, Translat Res Hematol Neoplasms Grp, Barcelona, Spain - Author
Lausanne Univ Hosp, Hematol Serv, Oncogen Lab, Lausanne, Switzerland - Author
MLL Munich Leukemia Lab, Munich, Germany - Author
Royal Bournemouth Hosp, Dept Mol Pathol, Bournemouth, Dorset, England - Author
Sorbonne Univ, Hop Pitie Salpetriere, AP HP, INSERM U1138, Paris, France - Author
St Anna Univ Hosp, Hematol Sect, Ferrara, Italy - Author
Univ Hosp Vall dHebron, Dept Hematol, Barcelona, Spain - Author
Univ Navarra, Dept Genet, Cytogenet & Hematol Genet Serv, Pamplona, Spain - Author
Univ Southampton, Fac Med, Canc Sci, Southampton, Hants, England - Author
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Abstract

Genome complexity has been associated with poor outcome in patients with chronic lymphocytic leukemia (CLL). Previous cooperative studies established five abnormalities as the cut-off that best predicts an adverse evolution by chromosome banding analysis (CBA) and genomic microarrays (GM). However, data comparing risk stratification by both methods are scarce. Herein, we assessed a cohort of 340 untreated CLL patients highly enriched in cases with complex karyotype (CK, 46.5%) with parallel CBA and GM studies. Abnormalities found by both techniques were compared. Prognostic stratification in three risk groups based on genomic complexity [0-2, 3-4 and ?5 abnormalities] was also analyzed. No significant differences in the percentage of patients classified into each category were detected, but only a moderate agreement was observed between methods when focusing in individual cases (?=0.507; p.

Keywords

cllfishkaryotypemutationsoutcomesrevealssurvivaltranslocationsAdultAgedArticleCell cycle assayChromosome 14Chromosome aberrationChromosome aberrationsChromosome bandingChromosome banding patternChronic lymphatic leukemiaClinical outcomeComparative genomic hybridizationConventional cytogeneticsCopy number variationCytogeneticsDisease free survivalFinite element analysisFluorescence in situ hybridizationGene rearrangementGenetic analysisGenetic variabilityGeneticsGenomicsHumanHumansImmunophenotypingIn situ hybridizationKaryotypeLeukemia, lymphocytic, chronic, b-cellLymphocytosisMajor clinical studyMaleMicroarray analysisMutationNon small cell lung cancerNucleic acid microarrayOverall survivalPolymerase chain reactionPrognosisProgression free survivalProspective studyRisk assessmentRna sequenceSequence analysisSomatic mutation

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Haematologica due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2022, it was in position 10/79, thus managing to position itself as a Q1 (Primer Cuartil), in the category Hematology.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 1.55. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 14, 2024)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Weighted Average of Normalized Impact by the Scopus agency: 1.56 (source consulted: FECYT Feb 2024)
  • Field Citation Ratio (FCR) from Dimensions: 5.49 (source consulted: Dimensions Jun 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-06-19, the following number of citations:

  • WoS: 15
  • Scopus: 17
  • Europe PMC: 9
  • OpenCitations: 18

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-19:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 25.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 25 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 4.75.
  • The number of mentions on the social network X (formerly Twitter): 3 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: France; Germany; Italy; Mali; Switzerland; United Kingdom; United States of America.