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Analysis of institutional authors

Melón-Ardanaz EAuthorGudiño VAuthorSalas AAuthor

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April 1, 2024
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Article

FixNCut: single-cell genomics through reversible tissue fixation and dissociation

Publicated to:Genome Biology. 25 (1): 81-81 - 2024-03-29 25(1), DOI: 10.1186/s13059-024-03219-5

Authors: Jimenez-Gracia, Laura; Marchese, Domenica; Nieto, Juan C; Caratu, Ginevra; Melon-Ardanaz, Elisa; Gudino, Victoria; Roth, Sara; Wise, Kellie; Ryan, Natalie K; Jensen, Kirk B; Hernando-Momblona, Xavier; Bernardes, Joana P; Tran, Florian; Sievers, Laura Katharina; Schreiber, Stefan; van den Berge, Maarten; Kole, Tessa; van der Velde, Petra L; Nawijn, Martijn C; Rosenstiel, Philip; Batlle, Eduard; Butler, Lisa M; Parish, Ian A; Plummer, Jasmine; Gut, Ivo; Salas, Azucena; Heyn, Holger; Martelotto, Luciano G

Affiliations

Adelaide Centre for Epigenetics (ACE), University of Adelaide, Adelaide, South Australia, Australia. - Author
Adelaide Centre for Epigenetics (ACE), University of Adelaide, Adelaide, South Australia, Australia. luciano.martelotto@adelaide.edu.au. - Author
Australian Genom Res Facil, Adelaide, SA, Australia - Author
Australian Genomics Research Facility, Adelaide, South Australia, Australia. - Author
Barcelona Inst Sci & Technol, Inst Res Biomed IRB Barcelona, Barcelona, Spain - Author
Cancer Immunology Program, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia. - Author
Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), Barcelona, Spain. - Author
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Barcelona, Spain. - Author
Centro Nacional de Análisis Genómico (CNAG), 08028, Barcelona, Spain. - Author
Centro Nacional de Análisis Genómico (CNAG), 08028, Barcelona, Spain. holger.heyn@cnag.eu. - Author
Ctr Invest Biomed Red Canc CIBERONC, Barcelona, Spain - Author
Ctr Invest Biomed Red Enfermedades Hepat & Digest, Barcelona, Spain - Author
Ctr Nacl Anal Genom CNAG, Barcelona 08028, Spain - Author
Department of Internal Medicine I, University Hospital Schleswig-Holstein (UKSH), Campus Kiel, Kiel, Germany. - Author
Department of Pathology & Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands. - Author
Department of Pulmonary Diseases, University of Groningen, University Medical Center Groningen, Groningen, Netherlands. - Author
Freemasons Foundation Centre for Men's Health, University of Adelaide, Adelaide, South Australia, Australia. - Author
Groningen Research Institute for Asthma and COPD, University of Groningen, University Medical Center Groningen, Groningen, Netherlands. - Author
ICREA, Barcelona, Spain - Author
ICREA, Barcelona, Spain. - Author
Inflammatory Bowel Disease Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain. - Author
Inst Invest Biomed August Pi & Sunyer IDIBAPS, Inflammatory Bowel Dis Grp, Barcelona, Spain - Author
Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Barcelona, Spain. - Author
Institute of Clinical Molecular Biology, Kiel University, Kiel, Germany. - Author
Monash Univ, Alfred Hosp, Dept Surg, Melbourne, Vic, Australia - Author
Monash University Department of Surgery, Alfred Hospital, Melbourne, VIC, Australia. - Author
Omniscope, Barcelona, Spain - Author
Omniscope, Barcelona, Spain. holger.heyn@cnag.eu. - Author
Omniscope, Barcelona, Spain. luciano.martelotto@adelaide.edu.au. - Author
Peter MacCallum Canc Ctr, Canc Immunol Program, Melbourne, Vic, Australia - Author
Sir Peter MacCallum Department of Oncology, The University of Melbourne, Melbourne, VIC, Australia. - Author
South Australian Health and Medical Research Institute, Adelaide, South Australia, Australia. - Author
South Australian Hlth & Med Res Inst, Adelaide, SA, Australia - Author
South Australian immunoGENomics Cancer Institute (SAiGENCI), University of Adelaide, Adelaide, South Australia, Australia. - Author
South Australian immunoGENomics Cancer Institute (SAiGENCI), University of Adelaide, Adelaide, South Australia, Australia. luciano.martelotto@adelaide.edu.au. - Author
St Jude Children's Research Hospital, Memphis, TN, 38105, USA. - Author
St Jude Childrens Res Hosp, Memphis, TN 38105 USA - Author
Univ Adelaide, Adelaide Ctr Epigenet ACE, Adelaide, SA, Australia - Author
Univ Adelaide, Freemasons Fdn Ctr Mens Hlth, Adelaide, SA, Australia - Author
Univ Adelaide, South Australian ImmunoGEN Canc Inst SAiGENCI, Adelaide, SA, Australia - Author
Univ Barcelona UB, Barcelona, Spain - Author
Univ Groningen, Univ Med Ctr Groningen, Dept Pathol & Med Biol, Groningen, Netherlands - Author
Univ Groningen, Univ Med Ctr Groningen, Dept Pulm Dis, Groningen, Netherlands - Author
Univ Groningen, Univ Med Ctr Groningen, Groningen Res Inst Asthma & COPD, Groningen, Netherlands - Author
Univ Hosp Schleswig Holstein UKSH, Dept Internal Med 1, Campus Kiel, Kiel, Germany - Author
Univ Kiel, Inst Clin Mol Biol, Kiel, Germany - Author
Univ Melbourne, Sir Peter MacCallum Dept Oncol, Melbourne, Vic, Australia - Author
Universitat de Barcelona (UB), Barcelona, Spain. - Author
Universitat de Barcelona (UB), Barcelona, Spain. holger.heyn@cnag.eu. - Author
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Abstract

The use of single-cell technologies for clinical applications requires disconnecting sampling from downstream processing steps. Early sample preservation can further increase robustness and reproducibility by avoiding artifacts introduced during specimen handling. We present FixNCut, a methodology for the reversible fixation of tissue followed by dissociation that overcomes current limitations. We applied FixNCut to human and mouse tissues to demonstrate the preservation of RNA integrity, sequencing library complexity, and cellular composition, while diminishing stress-related artifacts. Besides single-cell RNA sequencing, FixNCut is compatible with multiple single-cell and spatial technologies, making it a versatile tool for robust and flexible study designs.© 2024. Crown.

Keywords

AnimalAnimal tissueAnimalsArticleArtifactCell stressCellular stressColon tissueControlled studyDissociationDownstream processingFixncutGeneticsGenomicsHumanHuman cellHumansLung parenchymaMethodologyMiceMouseNonhumanProceduresReproducibilityReproducibility of resultsRnaRna sequencingSample fixationSequence analysis, rnaSingle cell analysisSingle cell rna seqSingle-cell analysisSingle-cell genomicsSpecimen handlingTissue dissociationTissue fixation

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Genome Biology due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 7/191, thus managing to position itself as a Q1 (Primer Cuartil), in the category Genetics & Heredity. Notably, the journal is positioned above the 90th percentile.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-08-12:

  • WoS: 3
  • Scopus: 3

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-08-12:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 53.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 53 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 48.7.
  • The number of mentions on the social network X (formerly Twitter): 91 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Australia; Germany; Netherlands; United States of America.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: Last Author (Martelotto LG).